A new approach to creating a biotechnology of molecular hydrogen (H2) production of organic waste (OW), based on the degradation of biopolymers by association of spore-forming microorganisms (Bacillus and Clostridium) was developed.
A selection of microorganisms which produce H2 has been conducted. The influence of mass transfer and regulation of microbial metabolism on the synthesis of H2 has been studied. Mass balance of hydrogen fermentation was calculated. The maximum content of H2 in the gas phase at 2-3 days amounted to 75% and the average content throughout the fermentation period - 60%, while total time of destruction (temporal index, T) - 5-7 days. From 1 kg of potatoes microbial association can synthesize up to 30 liters of hydrogen. Coefficient of destruction Kd and coefficient of efficiency of hydrogen production Kp were determined. Application RMM increases Kd from 2.7 to 5.8 units.
When cultured in agar medium with starch under anaerobic conditions (according to Hungate) strains were obtained, which hydrolyze starch and synthesize H2. With using of RMM obtained culture carried out the destruction of the potato with Kd = 9 and synthesized gas with a maximum concentration of H2 on 2d day to 80% and the average hydrogen content 60% during the whole period of fermentation.
Module pilot plant was tested and technological parameters of waste destruction and synthesis of H2 were calculated. From 1 kg of solid organic wastes 24 liters of gas is generated . The average concentration of hydrogen in the gas mixture was 40% (9.3 liters in total). Temporal index was 12 days.
Thus, theoretically developed and experimentally confirmed the possibility of obtaining molecular hydrogen while destruction of environmentally hazardous organic wastes.
Keywords: microbial synthesis of hydrogen, degradation of organic compounds, sporeforming bacteria